Endo-Beta-Galactosidase cleaves internal β(1-4) galactose linkages in unbranched, repeating poly-N-acetyllactosamine structures. Sulfated structures such as keratan sulfate are also cleaved. Branching and/or fucosylation of the substrate may decrease or eliminate cleavage. • Endo-Beta-Galactosidase is useful for identifying and removing poly-N-acetyllactosamine structures on many biologically important glycoconjugates. • Source: Recombinant from Bacteroides fragilis • EC 3.2.1.103 • CAS 55072-01-0 • Contents: 60 µl aliquot of enzyme (0.9 U) in 20 mM tris-HCl, pH 7.5 • 1 vial reaction buffer- 250mM Sodium phosphate, pH 5.8 • Specific Activity >140 U/mg • Activity >14 U/ml • Molecular weight ~32,000 daltons • Optimum pH 5.8