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New England Biolabs, Inc. Endonuclease IV – 5000 units
Supplier: New England Biolabs, Inc. M0304L
Endonuclease IV can act on a variety of oxidative damage in DNA. The enzyme is apurinic/apyrimidinic (AP) endonuclease that will hydrolyse intact AP sites in DNA. AP sites are cleaved at the first phosphodiester bond that is 5' to the lesion leaving a hydroxyl group at the 3' terminus and a deoxyribose 5'-phosphate at the 5' terminus. The enzyme also has a 3' -diesterease activity and can release phosphoglycolaldehyde, intact deoxyribose 5'-phosphate and phosphate from the 3' end of DNA.
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