Invitrogen™ Human Endoglin (CD105) ELISA Kit

Description

ELISA

The Human Endoglin ELISA quantitates Hu Endoglin in human serum, plasma, buffered solution, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu endoglin. Principle of the method The Human Endoglin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

Specifications

• Accession Number: P17813
• Assay Range: 12.35 to 3000 pg/mL
• Assay Sensitivity: 10 pg/mL
• Calibration Range: 10 pg/mL to 3000 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Product Type: ELISA
• Protein Family: artery morphogenesis; BMP signaling pathway; bone development; cell adhesion; cell chemotaxis; cell migration; cell migration involved in endocardial cushion formation; cell motility; cellular response to mechanical stimulus; central nervous system vasculogenesis; chronological cell aging; detection of hypoxia; extracellular matrix constituent secretion; extracellular matrix disassembly; heart looping; intracellular signal transduction; negative regulation of cell migration; negative regulation of endothelial cell proliferation; negative regulation of nitric-oxide synthase activity; negative regulation of pathway-restricted SMAD protein phosphorylation; negative regulation of protein autophosphorylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transforming growth factor beta receptor signaling pathway; patterning of blood vessels; positive regulation of BMP signaling pathway; positive regulation of collagen biosynthetic process; positive regulation of gene expression; positive regulation of pathway-restricted SMAD protein phosphorylation; positive regulation of protein phosphorylation; positive regulation of systemic arterial blood pressure; positive regulation of transcription from RNA polymerase II promoter; regulation of cell adhesion; regulation of cell proliferation; regulation of phosphorylation; regulation of transcription, DNA-templated; regulation of transforming growth factor beta receptor signaling pathway; response to corticosteroid; response to hypoxia; response to statin; response to transforming growth factor beta; smooth muscle tissue development; transforming growth factor beta-activated receptor activity; transforming growth factor beta receptor, cytoplasmic mediator activity; transforming growth factor beta receptor signaling pathway; transmembrane signaling receptor activity; vasculogenesis; venous blood vessel morphogenesis; wound healing
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with any of the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• Sufficient For: 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Colorimetric Microplate Reader
• Gene ID (Entrez): 2022
• Gene Symbol: END,HHT1,ORW1
• Interassay CV: <12%
• Intraassay CV: <10
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Research Discipline: Extracellular Matrix & Cell Adhesion
• Sample Volume: Plasma 5 μL, Serum 5 μL, Supernatant 50 μL
• Storage Requirements: 2°C to 8°C
• Synonym: RP11-228B15.2, END, HHT1, ORW1
• Target: CD105 (Endoglin)
• Test Time: 1 hrs 20 mins
• Total Assay Time: 4 hrs 45 mins