QA Bio Inc Endoglycosidase F1 - 200 uLs

Supplier:  QA Bio Inc EEF01200

Endo F1 cleaves Asparagine-linked high mannose and some hybrid oligosaccharides. Core fucosylation reduces the activity by 50 fold. Endoglycosidase F1 will hydrolyze sulfate containing high-mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. • Additional Endo F Products: Endoglycosidase F2 releases biantennary and high mannose glycans (at a 40X reduced rate) - Endoglycosidase F3 will release triantennarry and fucosylated biantennary N-glycans. • The Endo F Multi-Kit includes 20 µLs of each of the Endo F enzymes and their buffers. • Source recombinant Elizabethkingia miricola (was Chryseobacterium meningosepticum) in E. Coli • EC 3.2.1.96 • Contents: 60 µl aliquot of enzyme (1 U) in 20 mM Tris-HCl, pH 7.5 / 5x Reaction Buffer – 250 mM sodium phosphate, pH 5.5 • Specific Activity >16 U/mg • Activity >17 U/ml

Catalog No. 50-223-6644