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Cell Biolabs Inc Endothelial Tube Formation Assay (In Vitro Angiogenesis Assay), 50 assays
Supplier: Cell Biolabs Inc CBA200
One quick assessment of angiogenesis is the measurement of the ability of endothelial cells to form three-dimensional structures (tube formation). Endothelial cells form tubes on collagen or fibrin clot coated dishes after several days. Cell Biolabs’ Endothelial Tube Formation Assay Kit utilizes ECM (extracellular matrix) gel prepared from Engelbreth–Holm–Swarm (EHS) tumor cells. Endothelial tube formation on ECM gel closely mimics the in vivo environment and may be used to test angiogenesis inhibitors before in vivo testing. The Endothelial Tube Formation Assay Kit provides a robust system to assess angiogenesis in vitro. Tube formation can be achieved within 18 hours. Following staining the tube with the provided fluorescence dye, the extent of tube formation, such as average tube length and branch point, can be quantified through imaging software. Each kit provides sufficient quantities to perform up to 50 assays in 96-well plates.
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