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ENG Scientific Chlamydial Stain Kit
Description
- Kit Includes Solution I - Giemsa Stain 250 mL, Solution II - Buffer Solution 100 mL, and Solution III - Iodine Stain 100 mL
- Recommended Procedure:
- Giemsa Buffer Solution:
- 1 part of Sol. I to 40 or 50 parts of Sol. II. (If a darker stain is desired, increase ratio of stain to buffer.)
- Place fixed smear into Giemsa Buffer Sol. for 1 hour. (Time may vary according to intensity of stain desired.)
- NOTE: Giemsa Buffer Sol. should be freshly made.
- Slide is then rinsed rapidly in 95% ethanol to remove excess dye and to enhance differentiation.
- Iodine Method:
- Place fixed smear into Sol. III for 3 - 5 minutes.
- Slide is examined as a wet mount.
- NOTE: The slide may be decolorized with methyl alcohol and restained with Giemsa stain.
- Results:
- Giemsa Stain:
- The elementary bodies stain a reddish purple.
- The initial bodies are more basophilic, staining bluish as do bacteria.
- Iodine Stain:
- The matrix of inclusions may appear as a reddish-brown mass recognizable under low power.
- Check with higher objective to confirm.
- Giemsa Stain:
- Giemsa Buffer Solution:
- Contents: Giemsa Stain, Methanol, Buffer Salts, Iodine, Potassium Iodide
- These solutions are made from certified dyes (when applicable).
- For in vitro diagnostic use
- Chlamydial Stain Kit
- 1 Kit
Specifications
Specifications
| Type | Chlamydial Stain Kit |
| Quantity | Solution I: 1 x 250 mL, Solution II: 2 x 100 mL |
| Includes | 250 mL Solution I - Giemsa stain, 100 mL Solution II - buffer Solution and Solution III - iodine stain |
| For Use With (Application) | In vitro diagnostic use |
| Packaging Type | Complete Kit (Bottles) |
For in vitro diagnostic use only.
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