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ENG Scientific Leifson Bacterial Flagella Stain Kit
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Quantity:
4 x 250 mL
Description
- Kit Includes Solution I - Basic Fuchsin, (1.2%) Alcoholic 250 mL, Solution II - Tannic Acid (3%) 250 mL, Solution III - Sodium Chloride (1.5%) 250 mL, and Solution IV - Methylene Blue 250 mL
- Bacterial flagella are below the visual limit in size, approximately 0.1 to 0.3 μ in diameter.
- Flagella stains increase the width of these delicate appendages by forming a precipitate at the surface of the flagella making them visible with the optical microscope.
- The preparation may be used to establish polar or lateral distribution of flagella on a bacterial cell.
- Recommended Procedure:
- Preparation of Slides:
- Slides must be clean and grease free.
- To assure this, soak slides for 4 days in a slide cleaning solution (Product No. 6981).
- Thoroughly wash slides in distilled water and allow to dry.
- Working Solution:
- Mix equal parts of each solution (I, II, III) in a glass vessel and tightly stopper.
- Allow to stand 2 hours prior to use.
- NOTE: Working stain solution will stain flagella satisfactorily for only 2 - 3 days; therefore, only prepare as needed.
- This solution precipitates rapidly at room temperature.
- Use only the clear supernatant for staining.
- From a centrifuged 24 hour broth culture of a motile species of bacteria, a loopful of sediment is placed at the end of the slide.
- Tilt the slide allowing the liquid to run slowly to the other end and air dry.
- Quickly add 1 mL of stain mixture.
- Allow to stand 5 - 15 minutes at room temperature.
- When precipitate forms over the entire area of the slide, carefully wash off by flooding with water.
- Counterstain with 1:10 dilution of Sol. IV for 1 minute.
- Wash and drain dry.
- Preparation of Slides:
- Results: Flagella stain bright red.
- Contents: Basic Fuchsin, Ethanol, Tannic Acid, Sodium Chloride, Methylene Blue, HCl
- These solutions are made from certified dyes (when applicable).
- For in vitro diagnostic use.
- 1 Kit
Specifications
Specifications
Type | Leifson's Bacterial Flagella Stain Kit |
Quantity | 4 x 250 mL |
Includes | 250 mL Solution I - basic fuchsin, 250 mL (1.2%) alcoholic, 250 mL Solution II - tannic acid (3%), 250 mL Solution III - sodium chloride (1.5%), 250 mL Solution IV - methylene blue |
For Use With (Application) | In vitro diagnostic use |
Packaging Type | Complete Kit (Bottles) |
For in vitro diagnostic use only.
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