ENG Scientific Leifson Bacterial Flagella Stain Kit

Description

Leifson Bacterial Flagella Stain Kit is used to establish polar or lateral distribution of flagella on a bacterial cell. Includes Solution I - Basic Fuchsin, (1.2%) Alcoholic, Solution II - Tannic Acid (3%), Solution III - Sodium chloride (1.5%), and Solution IV - Methylene Blue.

• Kit Includes Solution I - Basic Fuchsin, (1.2%) Alcoholic 250 mL, Solution II - Tannic Acid (3%) 250 mL, Solution III - Sodium Chloride (1.5%) 250 mL, and Solution IV - Methylene Blue 250 mL
• Bacterial flagella are below the visual limit in size, approximately 0.1 to 0.3 μ in diameter.
• Flagella stains increase the width of these delicate appendages by forming a precipitate at the surface of the flagella making them visible with the optical microscope.
• The preparation may be used to establish polar or lateral distribution of flagella on a bacterial cell.
• Recommended Procedure:
  • Preparation of Slides:
    • Slides must be clean and grease free.
    • To assure this, soak slides for 4 days in a slide cleaning solution (Product No. 6981).
    • Thoroughly wash slides in distilled water and allow to dry.
  • Working Solution:
    • Mix equal parts of each solution (I, II, III) in a glass vessel and tightly stopper.
    • Allow to stand 2 hours prior to use.
    • NOTE: Working stain solution will stain flagella satisfactorily for only 2 - 3 days; therefore, only prepare as needed.
    • This solution precipitates rapidly at room temperature.
  • Use only the clear supernatant for staining.
  • From a centrifuged 24 hour broth culture of a motile species of bacteria, a loopful of sediment is placed at the end of the slide.
  • Tilt the slide allowing the liquid to run slowly to the other end and air dry.
  • Quickly add 1 mL of stain mixture.
  • Allow to stand 5 - 15 minutes at room temperature.
  • When precipitate forms over the entire area of the slide, carefully wash off by flooding with water.
  • Counterstain with 1:10 dilution of Sol. IV for 1 minute.
  • Wash and drain dry.
• Results: Flagella stain bright red.
• Contents: Basic Fuchsin, Ethanol, Tannic Acid, Sodium Chloride, Methylene Blue, HCl
• These solutions are made from certified dyes (when applicable).
• For in vitro diagnostic use.
• 1 Kit

Specifications

• Type: Leifson's Bacterial Flagella Stain Kit
• Quantity: 4 x 250 mL
• Includes: 250 mL Solution I - basic fuchsin, 250 mL (1.2%) alcoholic, 250 mL Solution II - tannic acid (3%), 250 mL Solution III - sodium chloride (1.5%), 250 mL Solution IV - methylene blue
• For Use With (Application): In vitro diagnostic use
• Packaging Type: Complete Kit (Bottles)

For in vitro diagnostic use only.