ENG Scientific Modified Trichrome Stain Kit

Description

For quick detection of Microsporidia Spores in Stool and Duodenal Aspirates. Non-evasive method for Modified Trichrome Stain Kit.

• This stain method was designed as a simple, non-invasive technique for the rapid detection of microsporidia spores in fecal material by light microscopy.
• The chromotrope based stain is a modification of the trichrome stain for intestinal protozoa.
• Recommended Procedure:
  • Specimens for light microscopial examination should be prepared from a suspension of unconcentrated liquid stool (10 μL) in Sol. I (1:3 ratio).
  • Smears are prepared directly and thinly spread on a glass slide covering a 45 x 25 mm area.
  • Fix smears in Sol. II for 5 minutes.
  • Stain smears with Sol. III for 90 minutes.
  • Rinse slides in Sol. IV for 10 seconds.
  • Rinse briefly in Sol. V to remove acid.
  • Dehydrate in Sol. V for 5 minutes, Sol. VI for 10 minutes and Sol. VII for 10 minutes.
  • Read microscopically under oil immersion lens (magnification x 1000).
• Results:
  • Enterocytozoon bienuesi spores appear ovoid and refractile at approximately 1.5 by 0.90 μm.
  • The spore wall of properly stained smears should appear a bright pinkish red.
  • Most bacteria and background debris stain a faint green.

Specifications

• Quantity: 2 x 250 mL
• Type: Modified Trichrome Solution V
• For Use With (Application): For the rapid detection of microsporidia spores in fecal material and duodenal aspirates by light microscopy

For in vitro diagnostic use only.