Invitrogen™ Mouse Eotaxin-2 (CCL24) ELISA Kit

Catalog No.	Quantity
EMCCL24X10	10 x 96 Tests

Catalog No. EMCCL24X10 Supplier Invitrogen™ Supplier No. EMCCL24X10

Description

ELISA

The Mouse Eotaxin-2 (CCL24) ELISA quantitates Ms Eotaxin-2 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Eotaxin-2. Principle of the method The Mouse Eotaxin-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Eotaxin 2 belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity on resting T lymphocytes, a minimal activity on neutrophils, and is negative on monocytes and activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line.

Specifications

• Accession Number: Q9JKC0
• Assay Range: 4-800 pg/mL
• Assay Sensitivity: 4 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Cytokines & Receptors
• Sample Type: Plasma, Serum, Supernatant
• Target Species: Mouse
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: CC chemokine CCL24; C-C motif chemokine 24; C-C motif chemokine ligand 24; Ccl24; chemokine (C-C motif) ligand 24; Ckb-6; CK-beta-6; Eosinophil chemotactic protein 2; Eotaxin2; eotaxin-2; eotaxin-2-like protein; MPIF2; MPIF-2; Myeloid progenitor inhibitory factor 2; Scya24; small inducible cytokine A24; small inducible cytokine subfamily A (Cys-Cys), member 24; small-inducible cytokine A24CC chemokine CCL24; C-C motif chemokine 24; C-C motif chemokine ligand 24; Ccl24; chemokine (C-C motif) ligand 24; Ckb-6; CK-beta-6; Eosinophil chemotactic protein 2; Eotaxin2; eotaxin-2; eotaxin-2-like protein; MPIF2; MPIF-2; Myeloid progenitor inhibitory factor 2; Scya24; small inducible cytokine A24; small inducible cytokine subfamily A (Cys-Cys), member 24; small-inducible cytokine A24; CKb-6; MPIF-2; Scya24
• Gene ID (Entrez): 56221
• Gene Symbol: CCL24
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Eosinophil chemotactic protein 2, Eotaxin-2, Small-inducible cytokine A24
• Quantity: 10 x 96 Tests
• Regulatory Status: RUO
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Target: Eotaxin 2
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min