Invitrogen™ Human EphB2, GST Tag Recombinant Protein

Catalog No.	Quantity
PV3625	10 μg

Catalog No. PV3625 Supplier Invitrogen™ Supplier No. PV3625

Description

Recombinant Protein

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 μg, 10 μg and 20 μg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 μL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase. Protein Form: Cytoplasmic.

EPHB2 is a receptor tyrosine kinase that belongs to the ephrin receptor family. Members of the Eph family of kinases play important roles in diverse biological processes including nervous system development, angiogenesis and neural synapsis formation and maturation. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB4 binds to ephrin-B2 and plays an essential role in vascular development.

Specifications

• Accession Number: P29323
• Concentration: See Label
• For Use With (Application): Kinase Assay
• Formulation: 50mM tris HCl with 0.5mM EDTA, 150mM NaCl, 0.02% Triton X-100, 2mM DTT, 50% glycerol and no preservative; pH 7.5
• Gene ID (Entrez): 2048
• Molecular Weight (g/mol): 58.5 kDa
• Name: Human EphB2, GST Tag
• pH Range: 7.5
• Purification Method: Purified
• Quantity: 10 μg
• Storage Requirements: -80°C, Avoid Freeze/Thaw Cycles
• Regulatory Status: RUO
• Gene Alias: CAPB; cEK5; chicken embryo kinase 5; chicken embryo kinase 5 protein; developmentally-regulated Eph-related tyrosine kinase; DRT; EFL6; EFNB3; EK5; ELK; ELK-related tyrosine kinase; embryo kinase 5 protein CEK5; EPH B2; EPH receptor B2; eph tyrosine kinase 3; EPHB2; EphB2/CTF1; EphB2/CTF2; EPHB3; EPH-like kinase 5; Ephrin B1; Ephrin B2; Ephrin B3; Ephrin B4; Ephrin type-B receptor 2; EphrinB1; EphrinB2; EphrinB3; EphrinB4; EPHT3; EPLG8; EPTH3; ERK; ETECK; ETK2; HEK2; Hek5; HEK-6; LERK8; MGC87492; NET; Neural kinase; Nuk; Nuk receptor tyrosine kinase; PCBC; Prkm5; protein-tyrosine kinase HEK5; Qek5; Renal carcinoma antigen NY-REN-47; RGD1564232; Sek3; TYRO5; TYRO6; Tyrosine-protein kinase receptor EPH-3; tyrosine-protein kinase receptor SEK-3; Tyrosine-protein kinase TYRO5
• Common Name: EphB2
• Gene Symbol: EPHB2
• Kinase Family: Ephrin & EPH Receptor Kinase Family
• Protein Length: aa 616-884
• Product Type: Protein
• Conjugate: Unconjugated
• Species: Human
• Recombinant: Recombinant
• Protein Tag: GST-tag
• Sequence: 616-884
• Content And Storage: Storage Buffer: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.5mM EDTA, 0.02% Triton X-100, 2mM DTT, 50% glycerol
  Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.
• Assay Entry: Biochemical phospho-specific activity (no antibody), Biochemical terbium phospho-specific activity, Biochemical europium phospho-specific activity, Biochemical binding/kinetic
• Kinase Group: Tyrosine Kinases
• Shipping Condition: Dry Ice
• System Compatibility: LanthaScreen™ Antibody, LanthaScreen™ Substrate, Zin-LYTE™, Omnia™ Bulk Substrate
• Target Entry: EPHB2
• Target Gene: EPH receptor B2
• Expression System: Insect cells
• Protein Family: Kinases & Inhibitors
• Protein Form: Recombinant, Catalytic Domain, Cytoplasmic
• Form: Liquid
• Protein Subtype: Receptor Tyrosine Kinases
• Research Category: Drug Discovery: Signal Transduction
• Purity or Quality Grade: 90% by SDS-PAGE