Invitrogen Epi5 Episomal iPSC Reprogramming Kit
Epi5 Episomal iPSC Reprogramming Kit
Manufacturer: Invitrogen A15960
DescriptionThe Epi5 Episomal iPSC Reprogramming Kit provides an easy-to-use, highly efficient set of 5 episomal vectors designed by Dr. Okita in the laboratory of Professor Yamanaka at the Center for iPS Cell Research and Application (CiRA), Kyoto University. This system produces transgene-free, virus-free human induced pluripotent stem cells (iPSCs) with efficiencies in the range of 0.04% to 0.3%, depending upon the cell type being reprogrammed.
Features of the Epi5 Episomal iPSC Reprogramming Kit include:
Create Transgene and Virus-free iPSCs
Episomal vectors are a well-described system for producing transgene-free, virus-free iPSCs, providing a source of iPSCs for all stages of your pluripotent stem cell research. Other reprogramming methods, such as lentivirus, contain transgenes that can integrate into the host genome, potentially disrupting the genome or causing unpredictable results.
As oriP/EBNA1 vectors, these episomal vectors contain 5 reprogramming factors (Oct4, Sox2, Lin28, Klf4, and L-Myc) and replicate extra-chromosomally only once per cell cycle. At this replication rate, the episomes are lost at a rate of approximately 5% per cell generation. This system shows enhanced iPSC generation through p53 suppression, and the inclusion of L-Myc has been shown to be more potent and specific then c-Myc during human iPSC generation (1).
This 2-vial kit provides the optimal system for episomal vector reprogramming and eliminates the need for use of small molecules in the reprogramming medium. The episomal vectors are introduced into the cell by electroporation. We recommend use of the NeonTransfection System for electroporation, allowing flexibility in the volume and thus number of cells needed for reprogramming.
Flexibility in Selection of Media Systems
The Epi5 Episomal iPSC Reprogramming Kit can be used with multiple cell types and media systems. Protocols for fibroblasts and CD34+ blood cells are available in either feeder or feeder-free based reprogramming.
1. Okita K, Matsumara Y, Sato Y, et al. A more efficient method to generate integration free human iPS cells. Nature Methods 8, 409-412, 2011.
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|Epi5™ Episomal iPSC Reprogramming Kit, 1 Kit, 8pH, Cag Promoter, Liquid, Transfection Delivery Method, Gateway Cloning Method, EBNA OriP Vector Type, Episomal Expression, Induced Pluripotent Stem Cell Generation, Multi-Gene Expression Function|
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For Research Use Only. Not for use in diagnostic procedures.