The Mouse Epidermal Growth Factor (EGF) ELISA Kit is designed to detect and quantify EGF levels in serum plasma and tissue homogenates with high precision This kit employs a sandwich ELISA technique offering a broad detection range of 7 8 pg/mL to 500 pg/mL and a sensitivity of 1 95 pg/mL ensuring accurate measurement of low-concentration samples The assay requires a sample volume of 50-100 L and typically delivers results within 1-5 hours optimized for efficiency in research settings Quantitative analysis is performed by measuring absorbance at 450 nm providing reliable data for studies involving EGF-related cellular processes wound healing or tissue regeneration Its standardized protocol ensures reproducibility across experimental conditions making it suitable for both basic research and preclinical applications