Gibco™ EpiLife™ Defined Growth Supplement (EDGS)

Catalog No.	Quantity
S0125	5 mL

Catalog No. S0125 Supplier Gibco™ Supplier No. S0125

Description

EpiLife™ Defined Growth Supplement (EDGS)

A defined, sterile, concentrated (100X) solution intended for use with EpiLife™ medium to culture human epidermal keratinocytes. EDGS is not intended for use with Medium 154. EDGS contains all of the growth factors and hormones necessary for the growth of human epidermal keratinocytes. Each bottle contains the correct amount of supplement for a 500 ml bottle of EpiLife™ medium. EDGS contains:

purified bovine serum albumin

purified bovine transferrin

hydrocortisone

recombinant human insulin-like growth factor type-1 (rhIGF-1)

prostaglandin E2 (PGE2)

recombinant human epidermal growth factor (rhEGF) The bovine products in EDGS have been isolated from animals of North American origin. The isolation process for these components includes steps that inactivate viruses. All components of EDGS are greater than 95% pure.

Specifications

• Content And Storage: minus 20 degrees C
• Cell Type: Keratinocyte Cells
• Concentration: 100 X
• Product Type: Growth Supplement
• Sterility: Sterile
• Product Line: Cascade Biologics, EpiLife
• Quantity: 5 mL
• Shipping Condition: Dry Ice
• Species: Human

Frequently Asked Questions (FAQs)

Are the Keratinocyte SFM basal medium and supplement kit available separately?

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

Which serum-free media can be used to culture mouse keratinocytes?

The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.

Which serum-free media can be used to culture human keratinocytes?

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.