Invitrogen™ Epinephrine/Adrenaline Competitive ELISA Kit

Catalog No.	Quantity
EEL158	96 Tests

Catalog No. EEL158 Supplier Invitrogen™ Supplier No. EEL158

Description

ELISA

The Epinephrine/Adrenaline (EPI) ELISA quantitates EPI in serum, plasma and other biological fluids. Principle of the method The Competitive multispecies ELISA research use only kit is designed to quantitatively measure The Epinephrine/Adrenaline (EPI) independent of species. A Epinephrine/Adrenaline standard is provided to generate a standard curve for the assay and all samples are read off a user generated standard curve.Standards or diluted samples are pipetted into a coated microtiter plate and Biotinylated Detection Ab specific to Epinephrine/Adrenaline is added. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each well and incubated. Then a TMB substrate solution is added to each well. The enzyme substrate reaction is terminated by the addition of stop solution and the color change is measured on a microplate reader. The concentration of Epinephrine/Adrenaline in the samples is then determined by comparing the OD of the samples to the standard curve. Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Adrenaline, also known as epinephrine, plays a critical role in the body's acute stress response and is synthesized from noradrenaline in catecholaminergic neurons. The biosynthesis involves four key enzymes. The process begins with tyrosine conversion into L-DOPA by tyrosine hydroxylase, followed by L-DOPA conversion to dopamine by aromatic L-amino acid decarboxylase. Dopamine is subsequently converted into noradrenaline by dopamine-beta-hydroxylase. The final step is the conversion of noradrenaline into adrenaline through phenylethanolamine N-methyltransferase (PNMT), which utilizes the cofactor S-adenosyl-L-methionine to catalyze the methylation. Adrenaline exerts its physiological effects by binding to adrenergic receptors, which belong to the G protein-coupled receptor (GPCR) superfamily. These receptors are integral to mediating the cardiovascular effects of catecholamines, contributing to increased heart rate, blood pressure, and energy mobilization. The presence of adrenaline in the central nervous system (CNS) accounts for a small portion of catecholamines, yet its function is significant in synaptic transmission and neuromodulation, as it facilitates synchronized acetylcholine release, thus impacting neuronal communication and muscle function.

Specifications

• Assay Range: 31.25-2000 pg/mL
• Assay Sensitivity: 18.75 pg/mL
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates, Plasma, Serum, Tissue Homogenates
• Target Species: All Species
• For Use With (Equipment): Absorbance Microplate Reader
• Interassay CV: <10%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 50 μL, Plasma 50 μL, Serum 50 μL, Tissue Homogenate 50 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Refer to product documentation for component specific storage temperature.
• Target: Adrenaline
• Test Time: 1 hr 20 min
• Total Assay Time: 2 hr 30 min