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Thermo Scientific™ EquiPhi29™ DNA Polymerase

Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution.

$100.65 - $1253.65

Specifications

For Use With (Application) MDA-WGA, RCA
Polymerase EquiPhi29 DNA Polymerase
Shipping Condition Dry Ice
Concentration 10 U/μL
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Products 3
Catalog Number Mfr. No. Quantity ProductName Price Quantity & Availability  
Catalog Number Mfr. No. Quantity ProductName Price Quantity & Availability  
A39390
Encompass_Preferred
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Thermo Scientific™
A39390
250 U
Each for $100.65

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Reg : $108.00

The following deviations and disclosures may produce prices below stated discounts but will not trigger price reductions: Price shown reflects a temporary, promotional price reduction. No other purchase or commercial obligation required to receive the reduced price. Price may change at any time. Promotional price valid on web orders only. Your contract pricing may differ. Other exclusions apply.
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A39391
Encompass_Preferred
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Thermo Scientific™
A39391
1000 U
Each for $339.65

Save 7%

Reg : $366.00

The following deviations and disclosures may produce prices below stated discounts but will not trigger price reductions: Price shown reflects a temporary, promotional price reduction. No other purchase or commercial obligation required to receive the reduced price. Price may change at any time. Promotional price valid on web orders only. Your contract pricing may differ. Other exclusions apply.
Learn More
Add to Cart
 
A39392
Encompass_Preferred
View Documents
Thermo Scientific™
A39392
5000 U
Each for $1,253.65

Save 10%

Reg : $1,388.00

The following deviations and disclosures may produce prices below stated discounts but will not trigger price reductions: Price shown reflects a temporary, promotional price reduction. No other purchase or commercial obligation required to receive the reduced price. Price may change at any time. Promotional price valid on web orders only. Your contract pricing may differ. Other exclusions apply.
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Add to Cart
 
Description

Description

Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution. This enzyme is significantly improved over phi29 DNA polymerase in protein thermostability, reaction speed, product yield, and amplification bias, while retaining all the benefits of the wild-type enzyme.

EquiPhi29 DNA Polymerase possesses strong strand displacement activity and 3'→5' proofreading exonuclease activity that acts preferentially on single-stranded DNA or RNA.

Features of EquiPhi29 DNA Polymerase
• Highest processivity and strand displacement activity—more than 70 kb long DNA stretches can be synthesized
• Low amplification bias
• Extremely high yields of amplified DNA, even from small amounts of template
• Highly accurate DNA synthesis in a short time 

Applications
EquiPhi29 DNA Polymerase may be used in a wide variety of applications including:
• Unbiased whole genome amplification (WGA)
• Rolling circle amplification (RCA)
• Protein-primed DNA amplification
• Cell-free cloning of lethal DNA
In situ genotyping with padlock probes
• RNA-primed DNA amplification

Notes
1. The addition of pyrophosphatase to the reaction mixture with EquiPhi29 DNA Polymerase may further enhance DNA synthesis
2. Because of the enzyme's 3'→5' proofreading exonuclease, 3’-modified primers are highly recommended.
3. EquiPhi29 DNA Polymerase can amplify DNA in a wide range of temperatures (30–45°C), with an optimal reaction temperature of 42°C.

Specifications

Specifications

MDA-WGA, RCA
EquiPhi29 DNA Polymerase
Dry Ice
10 U/μL
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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