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Sigma Aldrich Fine Chemicals Biosciences eSpCas9 GfP Protein from A
Supplier: Sigma Aldrich Fine Chemicals Biosciences ECAS9GFPPR250UG
Recombinant enhanced specificity Cas9-GfP protein from Streptococcus pyogenes (~192 KD) is a ready-to-use reagent for genome engineering experiments. When combined with target-specific guide RNAs eSpCas9-GfP protein will act as a targeted nuclease suitable for transfection of cell cultures and for the accelerated development of genetically-modified animals via one-cell embryo injection. An N-terminally fused enhanced green fluorescent protein with an excitation peak at 488 nm and emission peak at 509 nm allows for visualization of transfected RNP complex in addition to utility in flow cytometry applications. AThe protein also contains three varied nuclear localization sequences postioned for optimal activity.
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