Gibco™ Essential 8™ Flex Medium Kit

Description

Essential 8 Flex Medium is a xeno-free and feeder-free medium that supports the culture of pluripotent stem cells (PSCs) with a flexible feeding schedule.

Essential 8 Flex Medium is a xeno-free and feeder-free medium that supports the culture of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) without the need for weekend feeding. It is provided in a convenient two-component kit (500 mL basal medium and 10 mL supplement).

Essential 8 Flex provides all of the benefits of Essential 8 Medium: minimized variability of components, reliable and robust cultures with a xeno-free, cGMP produced medium, along with the added benefit of flexibility. Maintenance of standard PSCs no longer requires being in the lab seven days a week.

Flexibility

With Essential 8 Flex Medium, pluripotent stem cells (PSCs) need only be fed on the day of splitting and the day after. If the cells will be left without feeding for more than 48 hours, such as over a weekend, then simply double the feed volume on Friday.

Reliable and robust cultures

Essential 8 Flex Medium has been shown to support PSC growth for >30 passages without any signs of karyotypic abnormalities and to maintain the ability of iPSCs to differentiate into all three germ line lineages.

Cost effective

Essential 8 Flex Medium is provided in a convenient two-component kit (500 mL basal medium and 10 mL supplement), and when used with vitronectin (VTN-N), provides a cost effective, defined system for feeder-free culture of human pluripotent stem cells.

The Essential 8 Flex Medium formulation is based upon that published for Essential 8 Medium by Chen, et al., and allows for control and flexibility of PSC culture medium, without a negative impact to the health of the stem cells.

Order Info

Approved for shipment at Room Temperature or on Wet Ice

Specifications

• Cell Line: Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
• Cell Type: Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
• Classification: Xeno-free
• Concentration: Essential 8 Flex Basal Medium (1X), Essential 8 Flex Supplement (50X)
• Form: Liquid
• Product Type: Stem Cell Media
• Serum Level: Serum-free
• Sterility: Sterile-filtered
• Sterilization Method: Sterile-filtered
• With Additives: Phenol Red
• Manufacturing Quality: cGMP for medical devices, 21 CFR Part 820 and ISO 13485
• Product Line: Essential 8™
• Quantity: 1 Kit
• Shipping Condition: Supplement - dry ice, Basal - ambient
• Content And Storage: • 500 mL Basal Medium (store at 2–8°C and protect from light)
  • 10 mL Supplement (store at -5 to -20°C and protect from light)
• Culture Type: Adherent Cell Culture, Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic), Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)

For Research Use Only. Not for use in diagnostic procedures.