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Eurofins Discoverx PathHunter eXpress Flt3 Activity Assay, 2-Plate Kit. (93-0901E3CP16M)
Supplier: Eurofins Discoverx 930901E3CP16M
The PathHunter FLT3 Activity eXpress kit provides an easy-to-use cell based assay to study small molecule drug candidates targeting RTKs. This kit assesses RTK activity via detection of resulting FLT3 phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged FLT3 to the extracellular ligand binding domain of EGFR alongside an EA-tagged SH2 domain protein. Upon ligand induced receptor activation, cross-phosphorylation of the receptor leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
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