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Gibco™ Expi293™ Expression Medium
Description
Expi293™ Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression.
- Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
- Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine™ transfection reagent
- Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
- Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10 L in disposable bioreactors
- Does not contain phenol red
Expi293™ Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293™ Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.
Specifications
Specifications
| Cell Line | Expi293F™ |
| Classification | Animal Origin-free, Chemically-defined, Protein-free, Serum-free |
| Form | Liquid |
| Product Type | Expression Medium |
| With Additives | GlutaMAX |
| Without Additives | No Phenol Red |
| Product Line | Expi293™ |
| Quantity | 10 L |
| Content And Storage | One 10L Universal Bag Store in the dark at 2-8°C. |
Safety and Handling
For Research use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
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