Gibco™ Expi293™ MembranePro™ Expression System

Description

Expi293™ MembranePro™ Expression System

For researchers studying G protein–coupled receptors (GPCRs) and other membrane proteins, the Expi293™ MembranePro™ Expression System delivers enriched, functional membrane proteins with high efficiency, scalability and reliability for a wide variety of low or high throughput applications.

Features of the Expi293™ MembranePro™ Expression System include:

Efficient—more than 20-fold increase in membrane protein yield compared to the adherent culture system

Scalable—easy scale-up without the need for multiple flasks

Easy to use—no enzymatic or mechanical dissociation required

In addition, benefits of MembranePro™ technology include:

Proteins are displayed on human (or other mammalian) cell membranes

Cellular quality control and mammalian post-translational processing helps produce functional protein

Proteins bud off as lipoparticles and are easily collected from the culture medium

Lipoparticles are enriched with expressed receptors

With the less labor-intensive Expi293™ MembranePro™ Expression System protocol, you will:
1. Clone your gene of interest using a TOPO™ vector
2. Transfect a susupension culture of Expi293F™ cells using Expifectamine™ 293 transfection reagent
3. Harvest lipoparticles 48 hrs after addition of enhancers using MembranePro™ Precipitation Mix
4. Store lipoparticles at -80 C or proceed to downstream assays

Note: Exi293F™ Cells (A14527) and a pEF6 V5-His TOPO™ Expression Kit (K9610-20) are required for use of this system and must be purchased separately when needed.

Specifications

• Type: Expression System
• Sufficient For: 10 Reactions
• Delivery Type: Virus-Like Particles (VLPs)
• Quantity: 10 Reactions
• Content And Storage: • MembranePro™ Reagent, 1 x 0.3 mg (1 mg/mL), store at -5 to -30°C
  • MembranePro™ Precipitation Mix, 1 x 75 mL, store at room temperature
  • 1 ExpiFectamine™ 293 transfection kit (containing reagent and enhancers) for 1 L of culture, store at 2–8°C
  • Opti-MEM™ I Reduced Serum Medium, 1 x 100 mL, store at 2–8°C
  • Expi293™ Expression Medium, 1 x 1 L, store in the dark at 2–8°C
• Cell Line: Expi293F™
• Product Line: Expi293™, MembranePro™
• For Use With (Application): Membrane Protein Expression
• Promoter: EF-1α

For Research Use Only. Not for use in diagnostic procedures.