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Gibco™ Expi293™ Met (–) Protein Labeling Kit

Expi293 Met (-) Protein Labeling Kit includes Expi293 Met (-) Expression Medium and the ExpiFectamine 293 Met (-) Transfection Kit for rapid, high-yield transient protein expression from Expi293F cells for applications where labeling of methionine residues in proteins is desired

Supplier:  Gibco™ A41249


Catalog No. A41249

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Kit components are specifically formulated without methionine, allowing users to add labeled methionine of their choice


  • Methionine-free medium and transfection enhancer formulations
  • 2 to 10-fold higher protein yields compared to other transfection reagents used for high-density 293 cell culture
  • Same transient transfection protocol as ExpiFectamine 293 Transfection Kit
  • Scalable transfection from small to large scale while maintaining equivalent volumetric protein yields

Specifically designed to easily integrate with other components of the Expi293 Expression System, including Expi293F cells and Expi293 Expression Medium

Components included:

  • 1 L Expi293 Met (–) Expression Medium
  • 1 ExpiFectamine 293 Met (–) Transfection Kit sufficient to transfect 1 L of culture


Protein Labeling Kit
  • 3 x 0.9 mL ExpiFectamine 293 Transfection Reagent, store at 2°C to 8°CC
  • 5 mL ExpiFectamine 293 Enhancer 1, store at 2°C to 8°CC
  • 50 mL ExpiFectamine 293 Met (–) Enhancer 2, store at 2°C to 8°CC
  • 100 mL Opti-Plex Complexation Buffer, store at 2°C to 8°CC (protect from light)
  • 1 L Expi293 Met (–) Expression Medium, store at 2°C to 8°CC (protect from light)

Protein Production
Approved for shipment at Room Temperature or on Wet or Dry Ice


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For Research Use Only. Not for use in diagnostic procedures.