Gibco™ Expi293F™ Inducible GnTI- Cells

Description

Expi293F Inducible GnTI- cell line is derived from engineered Expi293F cells that do not have N-acetylglucosaminyltransferase I (GnTI) activity, therefore lacking complex N-glycans, and stably express the tetracycline repressor (TetR) protein, thus enabling tetracycline-regulated protein expression

Cell line established by the selective knockout of the GnTI gene and stable integration of the regulatory plasmid pcDNA6/TR, which encodes the TetR gene under the control of the human CMV promoter. It is a powerful tool for tetracycline-inducible high-yield expression of homogeneously glycosylated recombinant proteins.

Features:

• Suspension, high-density culture in Expi293 Expression Medium
• Homogeneous N-glycosylation of expressed proteins
• Tetracycline-inducible protein expression with extremely low basal expression levels
• Equivalent protein yields to parental Expi293F cells—up to 1 g/L

For inducible expression of aprotein of interest, pcDNA5/TO mammalian expresson vector or an equivalent inducible vector is required for tetracycline-regulated gene expression.

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 10⁷ viable cells/mL in Expi293 Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293 Expression Medium.

Specifications

• Content And Storage: 1 vial Expi293F Inducible GnTI- Cells (1x10⁷ cells/vial), store in vapor-phase liquid nitrogen
  
  
• Product Line: Expi293™
• Quantity: 1 vial
• Shipping Condition: Dry Ice

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.