Gibco™ ExpiFectamine™ 293 Met (–) Transfection Kit

Description

ExpiFectamine 293 Met (-) Transfection Kit is a core component of the Expi293 Protein Labeling Kit for rapid, high-yield transient protein expression from Expi293F cells for applications where labelling of methionine residues in proteins is desired

Kit components are specifically formulated without methionine, allowing users to add labeled methionine of their choice.

Featues:

• Methionine-free formulation
• 2- to 10-fold higher protein yields compared to other transfection reagents used for high-density 293 cell culture
• Same transient transfection protocol as ExpiFectamine 293 Transfection Kit
• Scalable transfection from small to large scale while maintaining equivalent volumetric protein yields

Specifically designed to work with Expi293F cell lines as part of the Expi293 Expression System

Components included in the ExpiFectamine 293 Met (–) Transfection Kit are:

• 2.7 mL ExpiFectamine 293 Reagent
• 5 mL ExpiFectamine 293 Transfection Enhancer
• 1 x 50 mL ExpiFectamine 293 Met (–) Transfection Enhancer 2
• 100 mL Opti-Plex Complexation Buffer (methionine-free)

Component volumes are sufficient to transfect 1 L of culture

Specifications

• Classification: AOF, Chemically Defined, Methionine-Free, Protein-Free, Serum Free
• Content And Storage: • 3 x 0.9 mL ExpiFectamine 293 Transfection Reagent, store at 2–8°C
  • 5 mL ExpiFectamine 293 Enhancer 1, store at 2–8°C
  • 50 mL ExpiFectamine 293 Met (–) Enhancer 2, store at 2–8°C
  • 100 mL Opti-Plex Complexation Buffer, store at 2–8°C (protect from light)
  
  
• Cell Type: Established Cell Lines
• For Use With (Application): Transfection
• Product Line: ExpiFectamine™
• Product Type: Transfection Kit
• Quantity: 1 Kit
• Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.