Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Gibco™ ExpiFectamine™ 293 Transfection Kit

Catalog No. A14525
Encompass_Preferred
Click to view available options
Quantity:
For 1 L of culture
For 10 L of culture
For 50 L of culture

Designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells.

The ExpiFectamine™ 293 Transfection Kit is a core component of the Expi293™ Expression System. It is designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells. The high-efficiency, cationic, lipid-based transfection reagent and transfection enhancers are designed to power the highest possible level of protein expression from Expi293F™ cells cultured in Expi293™ Expression Medium

  • Designed specifically for transfection of high density suspension cell culture, with matching transfection enhancers that boost transfection performance and protein expression
  • Achieves protein yields 2- to 10-fold higher than other transfection reagents used on high density 293 cell cultures
  • Employs the same transient expression protocols typically used in current low-density 293 suspension culture systems to easily switch from low density systems to the high yield, high density Expi293™ Expression System
  • Provides robust and reproducible transfection results, giving users greater confidence in their results
  • Scale transfections for culture volumes of less than 1 mL to greater than 10 liters, while maintaining equivalent volumetric protein yields

The ExpiFectamine™ 293 Transfection Kit is specifically designed to work with Expi293F™ cells in the Expi293™ Expression System. The transfection kit consists of the cationic lipid-based ExpiFectamine™ 293 Reagent and ExpiFectamine™ 293 Transfection Enhancers 1 and 2. The enhancers are added 18 to 22 hours post-transfection to boost transfection, cell viability, and protein expression. The protocol for use of ExpiFectamine™ 293 Transfection components is very similar to transfection protocols for other transient expression reagents, such as 293fectin™ and FreeStyle™ MAX.

TRUSTED_SUSTAINABILITY

Specifications

Cell Line Expi293F™
Classification Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Content And Storage 27 mL ExpiFectamine™ 293 Reagent
50 mL ExpiFectamine™ 293 Transfection Enhancer 1
500 mL ExpiFectamine™ 293 Transfection Enhancer 2

Sufficient for transfection of 10 L of culture

Store at +2°C to +8°C
Cell Type Established Cell Lines
Sample Type Plasmid DNA
Transfection Technique Lipid-based Transfection
For Use With (Application) Transfection
Product Line ExpiFectamine™
Product Type Transfection Kit
Purity Cell Culture Grade
Quantity For 10 L of culture
Serum Compatible No
Show More Show Less
WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

Product Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Your feedback has been submitted: Thank you for helping us improve our website.