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Gibco™ ExpiFectamine™ 293 Transfection Kit

Designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells.

$916.00 - $32920.00

Specifications

Cell Line Expi293F™
Classification Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Cell Type Established Cell Lines
Sample Type Plasmid DNA
Transfection Technique Lipid-based Transfection
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Products 3
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
A14525
Encompass_Preferred
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Gibco™
A14525
For 10 L of culture
Each for $7,720.00
Edge
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A14526
Encompass_Preferred
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Gibco™
A14526
For 50 L of culture
Each for $32,920.00
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A14524
Encompass_Preferred
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Gibco™
A14524
For 1 L of culture
Each for $916.00
Edge
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Description

Description

The ExpiFectamine™ 293 Transfection Kit is a core component of the Expi293™ Expression System. It is designed for transient transfection of high density cultures of HEK (human embryonic kidney) 293 cells. The high-efficiency, cationic, lipid-based transfection reagent and transfection enhancers are designed to power the highest possible level of protein expression from Expi293F™ cells cultured in Expi293™ Expression Medium

  • Designed specifically for transfection of high density suspension cell culture, with matching transfection enhancers that boost transfection performance and protein expression
  • Achieves protein yields 2- to 10-fold higher than other transfection reagents used on high density 293 cell cultures
  • Employs the same transient expression protocols typically used in current low-density 293 suspension culture systems to easily switch from low density systems to the high yield, high density Expi293™ Expression System
  • Provides robust and reproducible transfection results, giving users greater confidence in their results
  • Scale transfections for culture volumes of less than 1 mL to greater than 10 liters, while maintaining equivalent volumetric protein yields

The ExpiFectamine™ 293 Transfection Kit is specifically designed to work with Expi293F™ cells in the Expi293™ Expression System. The transfection kit consists of the cationic lipid-based ExpiFectamine™ 293 Reagent and ExpiFectamine™ 293 Transfection Enhancers 1 and 2. The enhancers are added 18 to 22 hours post-transfection to boost transfection, cell viability, and protein expression. The protocol for use of ExpiFectamine™ 293 Transfection components is very similar to transfection protocols for other transient expression reagents, such as 293fectin™ and FreeStyle™ MAX.

Specifications

Specifications

Expi293F™
Established Cell Lines
Lipid-based Transfection
ExpiFectamine™
Cell Culture Grade
Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Plasmid DNA
Transfection
Transfection Kit
No
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

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