Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Biochemical Reagents
  4. Nucleic Acid Introduction Reagents
  5. Transfection Reagents
  6. Gibco™ ExpiFectamine™ CHO Transfection Kit 

Gibco™ ExpiFectamine™ CHO Transfection Kit

Catalog No. A29129
Encompass_Preferred
Change view
Click to view available options
Quantity:
For 1 L of culture
For 10 L of culture
For 50 L of culture
3 product options available for selection
Product selection table with 3 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
A29129 For 1 L of culture
A29130 For 10 L of culture
A29131 For 50 L of culture
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 3 options available.
3 options
Catalog No. A29129 Supplier Gibco™ Supplier No. A29129
Only null left
Add to Cart
Edge
Add to Cart

Description

The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System, designed for transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells.

The ExpiFectamine CHO Transfection Kit is a core component of the ExpiCHO Expression System Kit (Cat. No. A29133) and enables transient transfection of high-density cultures of Chinese hamster ovary (CHO) cells. The high-efficiency, cationic transfection reagent, coupled with an optimized transfection enhancer and culture feed, will help power the highest possible level of protein expression from ExpiCHO-S Cells (Cat. No. A29127) cultured in ExpiCHO Expression Medium (Cat. No. A2910001).

All components work together synergistically with expression yields of 1–3 g per L of transfected culture demonstrated for some antibody and non-antibody proteins.

Features of the ExpiFectamine CHO Transfection Kit include:

  • Enables robust and reproducible transfection results, giving you greater confidence in your results and ability to use CHO cells from research to discovery
  • Achieve >90% transfection efficiency of high-density suspension CHO cell culture, with matching transfection enhancer and culture feed to boost transfection performance and protein expression
  • Uses one-third to one-half less plasmid DNA than other transfection reagents
  • Scale growth and transfection in culture formats from <1 mL in deep well plates up to 5 L when using Nalgene angled-bottom shake flasks
  • Flexible, reliable culture and transfection protocols with multiple options that readily fit into current transient expression workflows

The ExpiFectamine CHO Transfection Kit is specifically designed to work with ExpiCHO-S Cells in the ExpiCHO Expression System Kit. The transfection kit supplies the cationic ExpiFectamine CHO Transfection Reagent, ExpiFectamine CHO Transfection Enhancer, and ExpiCHO Feed. ExpiCHO-S Cells and ExpiCHO Expression Medium are available separately.

TRUSTED_SUSTAINABILITY

Specifications

Cell Line ExpiCHO-S™, CHO-S, SFM Adapted
Classification Animal Origin-free, Chemically-defined, Protein-free, Serum-free
Content And Storage Kit includes:
• ExpiFectamine™ CHO Reagent, 3.2 mL
• ExpiFectamine™ CHO Enhancer, 6 mL
• ExpiCHO Feed, 330 mL

Sufficient for transfection of 1 L of culture
Store in the dark at 2°C to 8°C.
Cell Type Ovarian Cells
Format Kit
Promoter pcDNA™ 3.4
Transfection Technique Lipid-based Transfection
Product Line ExpiFectamine
Product Type CHO Transfection Kit
Quantity For 1 L of culture
Shipping Condition Room Temperature
Show More Show Less

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.