Gibco™ ExpiSf™ Expression System Starter Kits

Description

The ExpiSf Expression System is a complete chemically-defined baculovirus-insect cell protein expression system that delivers superior yields and consistent performance run after run using a fast, streamlined workflow.

The ExpiSf Expression System offers

• A chemically defined, yeastolate-free, animal origin-free medium for consistent cell growth and protein expression
• Integrated components that work in concert to deliver three times higher protein yields compared to traditional insect expression platforms
• Robust production of high-titer, high-quality P0 recombinant baculovirus in suspension culture format without the need for further virus amplification
• A fast, simplified workflow that allows you to obtain protein in as little as 6–10 days; half the time of traditional protocols
• Scalable expression that can be applied to cultures from 24-deep well plates to >3 L shake flasks

Specifications

• Type: Insect Cell Protein Expression System
• Cloning Method: Restriction Enzyme/MCS
• Protein Tag: Untagged
• Delivery Type: Baculovirus
• Quantity: 1 kit
• Content And Storage:

  • 1000 mL bottle ExpiSf CD Medium, store in the dark at 2°C to 8°C
  • 4 x 1 mL ExpiSf Enhancer, 2°C to 8°C
  • 1 mL ExpiFectamine Sf Transfection Reagent, 2°C to 8°C
  • 2 x 1 vial ExpiSf9 Cells, store in vapor-phase liquid nitrogen
  • 500 mL Opti-MEM, 2°C to 8°C
  • 10 μg pFastBac1 vector, 2°C to 8°C
  • 4 ng pFastBac1-Gus control vector, 2°C to 8°C
  • 5 x 0.1 mL MAX Efficiency DH10Bac Competent Cells, -80°C
  • 24 x 125 mL Single-Use Nalgene PETG Erlenmeyer Flasks, store at room temperature
  
  

• Format: Kit with Erlenmeyer Flasks
• Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
• Cell Line: Sf9, SFM Adapted
• Product Line: ExpiSf™
• Promoter: Polyhedrin

For Research Use Only. Not for use in diagnostic procedures.