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Gibco™ ExpiFectamine™ Sf Transfection Reagent
Description
- Delivers robust and reproducible transfection, giving you greater confidence in your results
- Enables transfection of plasmid DNA in adherent and suspension Sf9 and Sf21 cell cultures for reliable recombinant baculovirus production
- Facilitates efficient production of high-titer, high-quality P0 recombinant baculovirus in suspension culture format without the need for further virus amplification
- Exhibits low toxicity and does not require medium change post-transfection
- Is part of a simple, fast protocol with a short 5-minutes complexation time
- Demonstrates linear scalability of transfections from culture volumes of 4mL to 500mL in suspension format
The ExpiFectamine Sf Transfection Reagent is a core component of the ExpiSf Expression System and has been optimized for transfection of ExpiSf9™ cells (Cat. No. A35243). However, the reagent also demonstrates superior performance using other Sf9 and Sf21 insect cells cultured in serum-free media such as Sf-900 II SFM or Sf-900 III SFM in both adherent and suspension culture formats.
Specifications
Specifications
| Content And Storage | Store at 2–8°C, do not freeze Shelf Life: 24 months from date of manufacture |
| Cell Type | Insect Cells |
| Sample Type | Baculovirus DNA, Plasmid DNA |
| Transfection Technique | Lipid-based Transfection |
| For Use With (Application) | Transfection |
| High-throughput Compatibility | High-throughput Compatible |
| Product Line | ExpiFectamine™ |
| Product Type | Transfection Reagent |
| Quantity | 1 mL |
| Serum Compatible | Yes |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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