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Gibco™ ExpiSf9™ Cells

Gibco™ ExpiSf9™ Cells are non-engineered derivative of Sf9 insect cells that have been adapted for high-density suspension growth in ExpiSf CD Medium.

Manufacturer:  Gibco™ A35243


Catalog No. A35243

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  • Enable consistent protein expression performance for over 20 passages after thaw
  • Allow you to attain yields of three times more protein per liter than other insect cells from baculovirus-infected culture as part of the ExpiSf Expression System
  • Achieve maximum cell densities of 2x107 cells per mL in ExpiSf CD Medium
  • Exhibit fast recovery post-thaw and doubling times of approximately 24 hours
  • Achieve high performance growth in multiple culture volumes from 4mL in deep-well plates to >3L in shake flasks

ExpiSf9 Cells are a core component of the ExpiSf Expression System. Frozen cells are supplied in a vial containing 1.5mL of cells at 1 x 107 viable cells per mL in ExpiSf™ CD Medium and 7.5% DMSO. The cells should be thawed in ExpiSf™ CD Medium.Cells are typically grown in shake flasks on a shaker platform in a non-humidified, non-CO2 cell culture incubator. Growing ExpiSf9 cells in adherent format for routine culture is not recommended.



1 vial (1.5 mL volume at 1.0 x 107 cells/mL), store in vapor-phase liquid nitrogen
Insect Cell
Spodoptera frugiperda
Sf9, SFM Adapted
Suspension Cell Culture
Dry Ice
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