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Gibco™ ExpiSf9™ Cells
Gibco™ ExpiSf9™ Cells are non-engineered derivative of Sf9 insect cells that have been adapted for high-density suspension growth in ExpiSf CD Medium.
Manufacturer: Gibco™ A35243
Description
- Enable consistent protein expression performance for over 20 passages after thaw
- Allow you to attain yields of three times more protein per liter than other insect cells from baculovirus-infected culture as part of the ExpiSf Expression System
- Achieve maximum cell densities of 2x107 cells per mL in ExpiSf CD Medium
- Exhibit fast recovery post-thaw and doubling times of approximately 24 hours
- Achieve high performance growth in multiple culture volumes from 4mL in deep-well plates to >3L in shake flasks
ExpiSf9 Cells are a core component of the ExpiSf Expression System. Frozen cells are supplied in a vial containing 1.5mL of cells at 1 x 107 viable cells per mL in ExpiSf™ CD Medium and 7.5% DMSO. The cells should be thawed in ExpiSf™ CD Medium.Cells are typically grown in shake flasks on a shaker platform in a non-humidified, non-CO2 cell culture incubator. Growing ExpiSf9 cells in adherent format for routine culture is not recommended.
Specifications
| 1 vial (1.5 mL volume at 1.0 x 107 cells/mL), store in vapor-phase liquid nitrogen | |
| Insect Cell | |
| Spodoptera frugiperda | |
| ExpiSf9™ |
| Sf9, SFM Adapted | |
| Suspension Cell Culture | |
| Cell | |
| Dry Ice |
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