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Edvotek™ Exploring the Genetics of Taste: SNP Analysis of the PTC Gene Using PCR Experiment Kit Products for Science Education

To identify the presence of the single nucleotide polymorphism (SNP) in an amplified segment of the PTC gene that links detection of the characteristic taste of PTC paper. Edvotek™ Exploring the Genetics of Taste: SNP Analysis of the PTC Gene Using PCR Experiment Kit includes 5 modules.

Manufacturer:  Edvotek™ 345

Catalog No. S29018


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Includes: Instructions, PCR EdvoBeads™, Universal DNA Buffer, TE Buffer, LyphoPrimer™ Mix, LyphoTemplate™ Control DNA, Dryzymes™ Restriction Enzyme HaeIII, Restriction Enzyme Dilution Buffer, 100 bp Ladder, Proteinase K, PTC Paper, Control Taste Paper, UltraSpec-Agarose, Electrophoresis Powder Buffer, InstaStain™ Ethidium Bromide, Disposable plastic cups, 15 ml Conical Tube, Snap-top microcentrifuge tubes, microcentrifuge tubes (1.5 ml screw-cap), 0.2 ml PCR tubes, wax beads, and salt packets

Requires : Thermal cycler, electrophoresis apparatus, power supply, automatic micropipet, microwave or hot plate, waterbath, UV transilluminator.

Description

Description

 Five Modules Includes:
  1. Extraction of DNA from buccal cells
  2. Amplifying the segment that contains the polymorphic nucleotide
  3. Digestion of the amplified fragment with the restriction enzyme that recognizes the SNP
  4. Analysis by gel electrophoresis
  5. Tasting the PTC paper to confirm the results obtained
  • For 25 reactions
  • Complete in 3 hrs. 30 min.
  • Level: Advanced
  • Requires wet ice shipment for next day delivery
Specifications

Specifications

Instructions, primer mix, DNA template, PCR EdvoBead™, DNA size ladder, Restriction Enzyme, Restriction Enzyme Reaction and Dilution Buffers, Lysis buffer, agarose, electrophoresis buffer, InstaStain™ Ethidium Bromide, PTC paper.
For 25 reactions
Complete in 3 hrs. 30 min.
Exploring the Genetics of Taste: SNP Analysis of the PTC Gene Using PCR Experiment Kit
Freezer Storage (Some components)
SDS
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Product Certifications
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