Edvotek™ Exploring the Genetics of Taste: SNP Analysis of the PTC Gene Using PCR Experiment Kit

Description

Includes

Instructions, PCR EdvoBeads™, Universal DNA Buffer, TE Buffer, LyphoPrimer™ Mix, LyphoTemplate™ Control DNA, Dryzymes™ Restriction Enzyme HaeIII, Restriction Enzyme Dilution Buffer, 100 bp Ladder, Proteinase K, PTC Paper, Control Taste Paper, UltraSpec-Agarose, Electrophoresis Powder Buffer, InstaStain™ Ethidium Bromide, Disposable plastic cups, 15 ml Conical Tube, Snap-top microcentrifuge tubes, microcentrifuge tubes (1.5 ml screw-cap), 0.2 ml PCR tubes, wax beads, and salt packets

Requires

Thermal cycler, electrophoresis apparatus, power supply, automatic micropipet, microwave or hot plate, waterbath, UV transilluminator.

To identify the presence of the single nucleotide polymorphism (SNP) in an amplified segment of the PTC gene that links detection of the characteristic taste of PTC paper. Edvotek™ Exploring the Genetics of Taste: SNP Analysis of the PTC Gene Using PCR Experiment Kit includes 5 modules.

Five Modules Include:

• Extraction of DNA from buccal cells
• Amplifying the segment that contains the polymorphic nucleotide
• Digestion of the amplified fragment with the restriction enzyme that recognizes the SNP
• Analysis by gel electrophoresis
• Tasting the PTC paper to confirm the results obtained

• For 25 reactions
• Complete in 3 hrs. 30 min.
• Level: Advanced
• Requires wet ice shipment for next day delivery

Specifications

• Product Type: Exploring the Genetics of Taste: SNP Analysis of the PTC Gene Using PCR Experiment Kit
• Grade: 9 to Undergraduate
• Includes: Instructions, primer mix, DNA template, PCR EdvoBead™, DNA size ladder, Restriction Enzyme, Restriction Enzyme Reaction and Dilution Buffers, Lysis buffer, agarose, electrophoresis buffer, InstaStain™ Ethidium Bromide, PTC paper.
• Quantity: 25 Reactions
• Time Range: Complete in 3 hrs. 30 min.
• Content And Storage: Freezer Storage (Some components)
• Class Size: 25 Students