EYFP mRNA encodes Enhanced Yellow Fluorescent Protein (EYFP) exhibitting increased brightness and a longer emission peak wavelength (excitation/emission at 527nm/514nm) enabling clear differentiation from other fluorescent proteins during multicolor fluorescence imaging EZ Cap EYFP mRNA (m1 ) has a concentration of approximately 1 mg/mL and expresses yellow fluorescent protein It is generated through co-transcriptional capping resulting in a Cap 1 structure The Cap 1 structure is more suitable for mammalian systems and exhibits higher transcriptional efficiency compared to Cap 0 structures (ARCA and mCap) The mRNA is modified with N1-Methylpseudo-UTP (m1 ) and contains a poly(A) tail which together suppress RNA-mediated innate immune responses and enhance mRNA stability and lifespan both in vitro and in vivo Additionally the poly(A) tail plays a crucial role in improving translation initiation efficiency