Thermo Scientific™ EZ-Link™ Amine-PEG3-Biotin

Description

Biotinylate proteins or surfaces using EDC or other amine-reactive crosslinkers with a mid-length (22.9A) biotinylation reagent containing a 3-unit polyethylene glycol spacer.

Thermo Scientific™ EZ-Link Amine-PEG³-Biotin is a biotinylation reagent that contains a 3-unit polyethylene glycol (PEG) spacer arm and a terminal primary amine for conjugation and protein labeling with EDC or other crosslinkers.

Amine-PEG2-Biotin is a water-soluble biotin compound containing polyethylene glycol (PEG) spacer arm and a terminal primary amine. The hydrophilic PEG spacer arm imparts water solubility that is transferred to molecules that are conjugated to the biotin compound. The PEG spacer arm also gives this reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules. The amine group of this amine-PEG-biotin compound can be reacted with the crosslinker EDC to form conjugates with carboxylates and 5' phosphates.

Highlights:

• Use the EDC crosslinker to couple this analog to carboxyl (-COOH) groups or 5' phosphate groups
• Water-soluble, polyethylene glycol (PEG) spacer arm
• Longer chain length for reduction of steric hindrance
• PEG spacer arm transfers hydrophilicity to final conjugate

Specifications

• Product Type: Amine-PEG3-Biotin
• Quantity: 50 mg
• Content And Storage: Store at 4°C.
• Product Line: EZ-Link™
• Cell Permeability: Cell-Impermeant
• Chemical Reactivity: Carboxylic Acid, Ketone, Aldehyde
• Reactive Moiety: Amine
• Solubility: Water
• Label or Dye: Biotin
• Spacer: Mid-length, Pegylated
• Label Type: Biotin & Analogs

For Research Use Only. Not for use in diagnostic procedures.