Thermo Scientific™ EZ-Link™ Hydrazide-PEG4-Biotin

Description

Biotinylate glycoproteins (aldehydes of oxidized sugars) with this mid-length (31.3A), pegylated biotinylation reagent containing a 4-unit PEG spacer for solubility.

Thermo Scientific™ EZ-Link Hydrazide-PEG⁴-Biotin (also called Biotin-PEG4-Hydrazide) is an aldehyde-reactive biotinylation reagent that contains a 4-unit polyethylene glycol (PEG) spacer arm for increased hydrophilicity.

Highlights:

• Carbohydrate-reactive - efficiently couples to oxidized poly- saccharide groups of glycoproteins at pH 4-6, resulting in stable hydrazone bonds

• Longest spacer arm of biotin-hydrazide reagents - minimizes steric hindrance for immunoprecipitation and other affinity purification methods involving protein interactions

• Water-soluble polyethylene glycol (PEG) spacer arm - confers hydrophilicity to final conjugate, preventing or minimizing aggregation of labeled proteins

Specifications

• Product Type: Hydrazide-PEG4-Biotin
• Quantity: 50 mg
• Content And Storage: Store at 4°C.
• Product Line: EZ-Link™
• Cell Permeability: Cell-Impermeant
• Chemical Reactivity: Carboxylic Acid, Ketone, Aldehyde
• Reactive Moiety: Amine, Hydrazide
• Solubility: DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
• Label or Dye: Biotin
• Spacer: Extra-long, Pegylated
• Label Type: Biotin & Analogs

For Research Use Only. Not for use in diagnostic procedures.