Thermo Scientific™ EZ-Link™ Hydrazide-PEG4-Desthiobiotin, No-Weigh™ Format

Description

Thermo Scientific™ EZ-Link™ Hydrazide-PEG4-Desthiobiotin No-Weigh™ Format is a pegylated, carbonyl-reactive labeling reagent whose biotin-like group is elutable from streptavidin, enabling its use for cell surface glycoprotein labeling and purification.

• Pegylated, carbonyl-reactive labeling reagent whose biotin-like group is elutable from streptavidin, enabling its use for cell surface glycoprotein labeling and purification.
• Desthiobiotin - analog of biotin allows easy elution from streptavidin, an ideal feature for affinity purification applications
• Glycoprotein labeling - tag glycosylated proteins at sialic acid residues to enable high recovery in pull-down assays with streptavidin beads
• Cell surface labeling - tag and isolate cell surface glycoproteins; reagent does not permeate membranes of whole cells
• Aldehyde-reactive - reacts with aldehydes formed by periodate-oxidation of sugar groups
• Hydrazide-activated - perform reactions at pH 4 to 6 in buffers such as sodium acetate
• Pegylated - spacer arm contains a hydrophilic, 4-unit, polyethylene glycol (PEG) group
• Enhances solubility - pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution

Specifications

• Product Type: Hydrazide-PEG4-Desthiobiotin
• Quantity: 5 x 1 mg
• Content And Storage: Store at 4°C.
• Labeling Method: Chemical Labeling
• Shipping Condition: Wet Ice
• Product Line: EZ-Link™
• Cell Permeability: Cell-Impermeant
• Chemical Reactivity: Carboxylic Acid, Ketone, Aldehyde
• Reactive Moiety: Amine, Hydrazide
• Solubility: DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
• Label or Dye: Desthiobiotin
• Spacer: Extra-long, Pegylated

For Research Use Only. Not for use in diagnostic procedures.