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Thermo Scientific™ EZ-Link™ Hydrazide-PEG4-Desthiobiotin, No-Weigh™ Format
Description
- Pegylated, carbonyl-reactive labeling reagent whose biotin-like group is elutable from streptavidin, enabling its use for cell surface glycoprotein labeling and purification.
- Desthiobiotin - analog of biotin allows easy elution from streptavidin, an ideal feature for affinity purification applications
- Glycoprotein labeling - tag glycosylated proteins at sialic acid residues to enable high recovery in pull-down assays with streptavidin beads
- Cell surface labeling - tag and isolate cell surface glycoproteins; reagent does not permeate membranes of whole cells
- Aldehyde-reactive - reacts with aldehydes formed by periodate-oxidation of sugar groups
- Hydrazide-activated - perform reactions at pH 4 to 6 in buffers such as sodium acetate
- Pegylated - spacer arm contains a hydrophilic, 4-unit, polyethylene glycol (PEG) group
- Enhances solubility - pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
Specifications
Specifications
| Product Type | Hydrazide-PEG4-Desthiobiotin |
| Quantity | 5 x 1 mg |
| Content And Storage | Store at 4°C. |
| Labeling Method | Chemical Labeling |
| Shipping Condition | Wet Ice |
| Product Line | EZ-Link |
| Cell Permeability | Cell-Impermeant |
| Chemical Reactivity | Carboxylic Acid, Ketone, Aldehyde |
| Reactive Moiety | Amine, Hydrazide |
| Solubility | DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water |
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For Research Use Only. Not for use in diagnostic procedures.
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