Thermo Scientific™ EZ-Link™ Maleimide Protein Labeling Kit

Description

A convenient method to label proteins with varying amounts of maleimide for crosslinking or conjugation with a thiol/sulfhydryl containing moiety. Sulfo-SMCC reacts with primary amines on a protein to form a covalently attached maleimide group that can be further reacted with a thiol/free cystine.

• A convenient method to label proteins with varying amounts maleimide for crosslinking or conjugation with a thiol/sulfhydryl containing moiety
• Sulfo-SMCC contained in this kit readily reacts with the primary amines on a protein to form a covalently attached maleimide group which can be further reacted with a reactive thiol or free cystine to create diverse bioconjugates
• Thermo Scientific™  Sulfo-SMCC is a heterobifunctional crosslinker which contains a sulfonated N-hydroxysuccinimide (NHS) ester and maleimide group that allow covalent conjugation of amine- and thiol/sulfhydryl-containing molecules
• Cyclohexane bridge confers added stability to the maleimide group making Sulfo-SMCC the ideal crosslinking agent for maleimide activation of proteins
• This kit contains sufficient reagents for five labeling reactions of up to 100 μL sample with a concentration of 1-10 mg/mL
• Sulfo-SMCC reagent readily dissolves in water

Specifications

• Quantity: 1 Kit
• Content And Storage: Contains 5 vials of sulfo-SMCC, 5 Zeba Dye & Biotin Removal columns, and BupH™ Phosphate Buffered Saline pack.
  
  Store sulfo-SMCC desiccated at –20°C, Zeba Dye and Biotin Removal columns at 2–8°C.
  
  
• Chemical Reactivity: Amine-to-sulfhydryl
• Labeling Method: Chemical Labeling
• Shipping Condition: Wet Ice
• Reactive Moiety: Sulfo-NHS, Maleimide
• Product Line: EZ-Link™