Thermo Scientific™ EZ-Link™ NHS-Biotin

Description

Biotinylate protein amines (e.g., lysines) with a simple, short (13.5A), NHS-ester biotinylation reagent containing a membrane-permeable spacer arm for intracellular labeling.

Thermo Scientific™ EZ-Link NHS-Biotin is the simplest NHS-ester activated biotinylation reagent for labeling primary amines (e.g., protein lysines), whose membrane permeability enables it to be used for general intracellular labeling.

Highlights:

• Forms stable amide bonds
• Reacts with primary amines at pH 7-9
• Must be dissolved in DMSO or DMF and can then be transferred to an aqueous reaction solution
• Contains a shorter spacer arm than NHS-LC-Biotin
• Penetrates cell membranes because it is uncharged

Specifications

• Product Type: NHS-Biotin
• Quantity: 100 mg
• Content And Storage: Store desiccated at room temperature.
• Product Line: EZ-Link™
• Cell Permeability: Cell-Permeant
• Chemical Reactivity: Amine
• Reactive Moiety: Active Ester, Succinimidyl Ester, NHS Ester
• Solubility: DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
• Label or Dye: Biotin
• Spacer: Short
• Label Type: Biotin & Analogs

For Research Use Only. Not for use in diagnostic procedures.