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Thermo Scientific™ EZ-Link™ NHS-PEG4 Biotinylation Kit

Biotinylate protein amines and maximize solubility of proteins with this long (29.0A) NHS-ester biotinylation reagent containing a pegylated, water-soluble spacer arm.

Supplier:  Thermo Scientific™ 21455

Catalog No. PI21455

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Thermo Scientific™ EZ-Link NHS-PEG&subscript_4;-Biotin is a pegylated, water-soluble reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing macromolecules.


  • Water-soluble polyethylene glycol (PEG) spacer arm increases solubility of labeled protein
  • Prevents aggregation of antibodies better than Sulfo-NHS-LC-Biotin
  • Labels at N-terminus or lysine residues in less than 1 hour
  • Thermo Scientific No-Weigh Packaging (8 x 2mg) saves time - preserves reagent activity and prevents cross-contamination between use


EZ-Link NHS-PEG4 Biotinylation Kit
8 Reaction Kit
1 to 10 mg
NHS-PEG4 Biotinylation Kit
Sufficient For: 8 labeling reactions, each with 1 to 10 mg of antibody
• EZ-Link NHS-PEG4-Biotin, 8 x 2 mg
• PBS Pack (makes 500mL), 1 pack
• Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns
• HABA Solution, 1 mL
• Avidin, 10mg

Store avidin and microtubes of biotin reagent at -20°C. Store PBS at room temperature. Store remaining kit components at 4°C.
Antibodies, Proteins
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Safety and Handling

Safety and Handling

WARNING: Cancer -


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For Research Use Only. Not for use in diagnostic procedures.