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Thermo Scientific™ EZ-Link™ Psoralen-PEG3-Biotin

Catalog No. PI29986
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5 mg
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PI29986 5 mg
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Catalog No. PI29986 Supplier Thermo Scientific™ Supplier No. 29986
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Description

Biotinylate DNA or RNA via intercalation and conjugation with thymine- and other pyrimidine-containing bases with this light-activated biotinylation reagent.

Thermo Scientific™ EZ-Link Psoralen-PEG3-Biotin enables biotinylation of DNA or RNA via UV-light-activated intercalation of the psoralen group with thymine- and other pyrimidine-containing bases to form covalent bonds.

Highlights:

  • Water-soluble, polyethylene glycol (PEG) spacer arm
  • Much higher coupling yield than achieved with photoactivatable biotin
  • Psoralen moiety reacts through a cyclo-addition mechanism with the 5,6 double bond in thymine- and other pyrimidine-containing bases
  • Covalent attachment occurs with exposure to UV light (approximately equal to 350nm)
  • Great for biotinylating DNA/RNA probes (∽1 biotin/10-20 bases)
  • Ultrahigh sensitivity relative to radiolabeled probes (<100 femtograms)
  • Does not interfere with hybridization
TRUSTED_SUSTAINABILITY

Specifications

Product Type Psoralen-PEG3-Biotin
Quantity 5 mg
Content And Storage Store at 4°C protected from light and moisture. Shipped at ambient temperature.
Product Line EZ-Link
Cell Permeability Cell-Impermeant
Chemical Reactivity Light-activated
Reactive Moiety Psoralen
Solubility DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
Label or Dye Biotin
Spacer Extra-long, Pegylated
Label Type Biotin & Analogs
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Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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