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Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin
GSA_VA

Catalog No. PI21217
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PI21217 50 mg
PIA39256 10 x 1 mg
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Catalog No. PI21217 Supplier Thermo Scientific™ Supplier No. 21217
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Description

Thermo Scientific™ EZ-Link™ Sulfo-NHS-Biotin is a short-chain, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Label antibodies, proteins and other molecules that have primary amines with Thermo Scientific™ EZ-Link Sulfo-NHS-Biotin, a short-chain, water-soluble biotinylation reagent.

  • Protein labeling - biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
  • Cell surface labeling - biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
  • Amine-reactive - reacts with primary amines (-NH2), such as lysine side-chains or the amino-termini of polypeptides
  • Soluble - charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
  • Irreversible - forms permanent amide bonds; spacer arm cannot be cleaved
  • Very short - spacer arm (total length added to target) is 13.5 angstroms; it consists of the native biotin valeric acid group only

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TRUSTED_SUSTAINABILITY

Specifications

Description EZ-Link Sulfo-NHS-Biotin
Product Type Sulfo-NHS-Biotin
Quantity 50 mg
Content And Storage Store desiccated at -20°C. Shipped at ambient temperature.
Product Line EZ-Link
Cell Permeability Cell-Impermeant
Chemical Reactivity Amine
Reactive Moiety Active Ester, Succinimidyl Ester, Sulfo-NHS Ester
Solubility DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
Label or Dye Biotin
Spacer Short
Label Type Biotin & Analogs
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Frequently Asked Questions (FAQs)

Can you provide the shelf-life for EZ-Link Sulfo-NHS-Biotin?

EZ-Link Sulfo-NHS-Biotin is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (-20 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
Why would I chose a Sulfo-NHS Biotin over its non-sulfonated counterpart?

The sodium sulfonate group lends water solubility to the reagent.
Note: The actual biotin moiety that is added is identical between NHS and Sulfo-NHS biotins that vary only in the addition of the sodium sulfonate on the leaving group. Researchers preferring a more water soluble biotin be added to their molecule should consider a PEGylated version of biotin.

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

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