Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin

Description

Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Thermo Scientific™ EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

• Protein labeling - biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface labeling - biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
• Amine-reactive - reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
• Soluble - charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible - forms permanent amide bonds; spacer arm cannot be cleaved
• Medium length - spacer arm (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass

Specifications

• Product Type: Sulfo-NHS-LC-Biotin
• Quantity: 10 x 1 mg
• Content And Storage: Store desiccated at -20°C.
• Labeling Method: Chemical Labeling
• Shipping Condition: Qualified for Ambient or Wet Ice
• Product Line: EZ-Link™
• Cell Permeability: Cell-Impermeant
• Chemical Reactivity: Amine
• Reactive Moiety: NHS Ester
• Solubility: DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
• Label or Dye: Biotin
• Spacer: Mid-length

For Research Use Only. Not for use in diagnostic procedures.