Learn More
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin
Description
Thermo Scientific™ EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
- Protein labeling - biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
- Cell surface labeling - biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
- Amine-reactive - reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides
- Soluble - charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
- Irreversible - forms permanent amide bonds; spacer arm cannot be cleaved
- Medium length - spacer arm (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass
Specifications
Specifications
| Product Type | Sulfo-NHS-LC-Biotin |
| Quantity | 10 x 1 mg |
| Content And Storage | Store desiccated at -20°C. |
| Labeling Method | Chemical Labeling |
| Shipping Condition | Qualified for Ambient or Wet Ice |
| Product Line | EZ-Link |
| Cell Permeability | Cell-Impermeant |
| Chemical Reactivity | Amine |
| Reactive Moiety | NHS Ester |
| Solubility | DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water |
| Show More |
Frequently Asked Questions (FAQs)
No, the EZ-Link Sulfo-NHS-LC-Biotin, No-Weigh Format (Cat. No. A39257) should not be dissolved in water for storage. We recommended storing EZ-Link Sulfo-NHS-LC-Biotin, No-Weigh Format at -20 degrees C with desiccant as it is very moisture sensitive. Furthermore, to avoid moisture condensation onto the product, equilibrate vial to room temperature before opening.
EZ-Link Sulfo-NHS-LC-Biotin is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (-20 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.
EZ-Link Sulfo-NHS-LC-Biotin, No-Weigh Format is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (desiccated, at -20 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).
The No-Weigh product format, where small amounts of reagent are pre-weighed and packaged separately, prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial. This format enables use of a fresh vial of reagent each time and eliminates the hassle of weighing out small amounts of reagent. Please view additional information and the video at: https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/no-weigh-packaging.html
For Research Use Only. Not for use in diagnostic procedures.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.