Thermo Scientific™ EZ-Link™ Micro Sulfo-NHS-LC-Biotinylation Kit

Catalog No.	Quantity
PI21935	8 Reactions

Catalog No. PI21935 Supplier Thermo Scientific™ Supplier No. 21935

Description

Biotinylate protein amines (e.g., lysines) with this very popular mid-length (22.4A), water-soluble, NHS-ester biotinylation reagent for antibody or cell-surface labeling.

Thermo Scientific™ EZ-Link Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.

Highlights:

• Water-soluble analog of NHS-LC-Biotin
• Reacts with primary amines at pH 7-9 in non-amine-containing buffers such as borate, carbonate or phosphate buffers
• Negative charge of -SO₃ group keeps biotinylation localized at the cell surface
• Creates an amide bond

U.S. Patents 5,892,628 and 5,872,261.

Specifications

• Description: EZ-Link Micro Sulfo-NHS-LC-Biotinylation Kit
• Product Type: Micro Sulfo-NHS-LC-Biotinylation Kit
• Quantity: 8 Reactions
• Content And Storage: Sufficient For: 8 labeling reactions, each with 0.05–0.2 mg of antibody
  • EZ-Link Sulfo-NHS-LC-Biotin, 8 x 1 mg
  • PBS Pack (makes 500 mL), 1 pack
  • Zeba Spin Desalting Columns (7K MWCO), 2 mL, 10 columns
  
  Store biotin reagent at -20°C. Store remaining kit components at 4°C.
• Labeling Scale: 100 μg
• Product Line: EZ-Link
• Solubility: Water
• Label or Dye: Biotin
• Labeling Target: Antibodies, Proteins

For Research Use Only. Not for use in diagnostic procedures.