Mito-EGFP mRNA encodes a fusion protein composed of a mitochondrial localization sequence fused with enhanced green fluorescent protein (EGFP) Through fusion of EGFP with a mitochondrial-targeting signal it allows real-time observation of mitochondrial dynamics in living cells aiding in revealing mitochondria s key roles in energy metabolism signal transduction and apoptosis Due to its high fluorescence efficiency and mitochondria-specific localization Mito-EGFP simplifies mitochondrial visualization facilitating a clearer understanding of mitochondrial function and behavior in cells EZ Cap Mito-EGFP Probe mRNA (m1 ) is provided at 1 mg/mL and is specifically optimized for mitochondria-specific expression of green fluorescent protein labeling This mRNA is synthesized through co-transcriptional capping resulting in a Cap 1 structure The incorporation of m1 modifications poly(A) tail significantly enhances mRNA stability and lifetime both in vitro and in vivo