Invitrogen™ F(ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, FITC, eBioscience™

Catalog No.	Quantity
50-112-8677	25 μg
50-112-2113	100 μg

Catalog No. 50-112-8677 Supplier Invitrogen™ Supplier No. 11401080

Description

F(ab')2-Goat Polyclonal Secondary Antibody

Description: The goat anti-mouse IgG polyclonal antibody reacts with the heavy and light chains of mouse IgG, with minimal cross reactivity to human, rat, bovine, goat, hamster, and rabbit serum proteins. Reactivity has been shown to IgG1, IgG2a, IgG2b, and IgG3, as well as some reactivity to IgM. The antibody is a F(ab')2 fragment which contains the 2 Fab domains still linked through the hinge region of the immunoglobulin. Removal of the Fc region helps to reduce issues typically found with Fc-mediated binding. This polyclonal has been validated as a secondary reagent (its ability to recognize cells stained with a mouse IgG followed by this polyclonal antibody). Applications Reported: This polyclonal antibody has been reported for use in flow cytometric analysis, immunohistochemistry, and immunocytochemistry. Applications Tested: This polyclonal antibody has been tested by flow cytometric analysis of normal human peripheral blood cells stained first with a mouse primary antibody. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This polyclonal antibody has been tested by immunocytochemistry on fixed and permeabilized HeLa cells that have been stained first with a mouse anti-human antibody. This can be used at less than or equal to 20 μg/mL. It is recommended...

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Mouse IgG (H+L)
• Applications: Flow Cytometry, Immunohistochemistry, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.5 mg/mL
• Conjugate: FITC
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Host Species: Goat
• Purification Method: Affinity chromatography
• Quantity: 25 μg
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Secondary Antibody
• Form: Liquid
• Isotype: Ig