Invitrogen™ F(ab')2-Goat anti-Rabbit IgG (H+L) Secondary Antibody, NANOGOLD™

Catalog No.	Quantity
N24916	1 mL

Catalog No. N24916 Supplier Invitrogen™ Supplier No. N24916

Description

F(ab')2-Goat Polyclonal Secondary Antibody

The NANOGOLD conjugate of Fab' fragment of goat anti-rabbit IgG is labeled with small uniformly sized (1.4 nm) gold particles at a ~1:1 ratio in the hinge region, remote from the antigen-binding site and can be used in immunoblotting, light microscopy, and electron microscopy applications.

Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Rabbit IgG (H+L)
• Applications: Immunomicroscopy, Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Nanogold
• Formulation: PBS with 0.1% BSA and 0.05% sodium azide; pH 7.4
• Host Species: Goat
• Immunogen: Gamma Immunoglobin.
• Purification Method: Purified
• Quantity: 1 mL
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Target Species: Rabbit
• Content And Storage: 4°C
• Product Type: Secondary Antibody
• Form: Liquid
• Isotype: IgG

Frequently Asked Questions (FAQs)

I want to label F-actin with phalloidin and image with transmission electron microscopy. What is the best way to go about it?

One way is to use a biotin-phalloidin conjugate, then label that with a colloidal gold conjugate of streptavidin. You will need to block endogenous biotin first. Another method is to label with fluorescein phalloidin, then with an anti-fluorescein antibody conjugated with colloidal gold.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov