Invitrogen™ F(ab)-Goat anti-Mouse IgG1 Superclonal™ Secondary Antibody

Catalog No.	Quantity
SA510392	250 μL

Catalog No. SA510392 Supplier Invitrogen™ Supplier No. SA510392

Description

F(ab)-Goat Recombinant Superclonal Secondary Antibody

F(ab)-Goat anti-Mouse IgG1 Superclonal™ Recombinant Secondary Antibody is very specific to Mouse IgG1 with no cross reactivity to Mouse IgG2a, Mouse IgG2b and Mouse IgG3. It showed minimum cross reactivity to Rabbit IgG, Rat IgA and no cross-reactivity to various species immunoglobulins tested. This Fab fragment antibody is expressed in vitro and purified as a recombinant construct. It is also engineered to deliver enhanced stability and sensitivity. The advantages of recombinant antibodies include-better specificity, animal origin-free formulation, and lot-to-lot consistency. F(ab) fragments offer several advantages over full-length IgGs due to their smaller molecular size (~50 kDa vs. ~150 kDa), such as improved tissue penetration. The absence of the Fc region reduces nonspecific binding and background staining in Fc receptor-expressing cells and tissues, while also enabling increased flexibility in multiplexing assays. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Specifications

• Antigen: Mouse IgG1
• Applications: ELISA, Western Blot, Immunocytochemistry
• Classification: Recombinant Superclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide; pH 7.4
• Host Species: Goat
• Immunogen: Purified Mouse IgG.
• Purification Method: Affinity chromatography
• Quantity: 250 μL
• Regulatory Status: RUO
• Primary or Secondary: Secondary
• Target Species: Mouse
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Secondary Antibody
• Form: Liquid
• Isotype: IgG F(ab)