Invitrogen™ Human FABP2 (intestinal) ELISA Kit

Catalog No.	Quantity
EHFABP2X5	5 x 96 Tests
EHFABP2	96 Tests

Catalog No. EHFABP2X5 Supplier Invitrogen™ Supplier No. EHFABP2X5

Description

ELISA

The Human FABP2 (intestinal) ELISA quantitates Hu FABP2 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FABP2. Principle of the method The Human FABP2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

The FABP2 gene encodes the intestinal fatty acid-binding protein 2 (I-FABP), which plays a significant role in lipid metabolism by facilitating the transport and intracellular trafficking of long-chain fatty acids in enterocytes, the absorptive cells in the intestine. FABP2 is involved in the uptake, metabolism, and transfer of fatty acids across cellular membranes, contributing to digestion and absorption processes in the intestine. This protein is crucial for efficient lipid metabolism, influencing fatty acid oxidation, and energy balance. Variations in the FABP2 gene have been associated with metabolic traits, such as insulin sensitivity, lipid profiles, and obesity, suggesting its impact on metabolic health. FABP2's function in intestinal lipid absorption and metabolism makes it a subject of interest in studies related to dietary effects on health and the development of metabolic disorders. Understanding FABP2's role is essential for insights into nutritional influences on health and possible therapeutic targets for metabolic diseases.

Specifications

• Accession Number: P12104
• Assay Range: 25-100 ng/mL
• Assay Sensitivity: 25 pg/mL
• Calibration Range: 25 pg/mL to 100 ng/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Other Proteins
• Sample Type: Plasma, Serum, Supernatant
• Specificity: In this ELISA antibody pair format, no cross-reactivity was observed with rhFABP-1, 3, 5, 6, 7, 8 and 9.
• Sufficient For: 5 x 96 Assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: FABP; Fabp2; Fabpi; fatty acid binding protein 1; fatty acid binding protein 2; fatty acid binding protein 2, intestinal; fatty acid-binding protein 2; Fatty acid-binding protein, intestinal; I-FABP; intestinal fatty acid binding protein; intestinal fatty acid binding protein 2; intestinal-type fatty acid-binding protein; MGC133132FABP; Fabp2; Fabpi; fatty acid binding protein 1; fatty acid binding protein 2; fatty acid binding protein 2, intestinal; fatty acid-binding protein 2; Fatty acid-binding protein, intestinal; I-FABP; intestinal fatty acid binding protein; intestinal fatty acid binding protein 2; intestinal-type fatty acid-binding protein; MGC133132;
• Gene ID (Entrez): 2169
• Gene Symbol: FABP2
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Fatty acid-binding protein 2, Intestinal-type fatty acid-binding protein, I-FABP
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Other Research
• Sample Volume: Plasma 50 μL, Serum 50 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: FABPI, I-FABP
• Target: FABP2
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min