This ELISA kit is designed for the quantitative detection of FAS (Factor-related Apoptosis) in mouse serum plasma cell culture supernates and tissue homogenates using a sandwich assay methodology The kit demonstrates a detection range spanning from 0 625 ng/mL to 40 ng/mL with a sensitivity threshold of 0 156 ng/mL enabling precise measurement of low-abundance targets Employing a straightforward protocol requiring 50-100 L of sample per test results can be obtained within 1-5 hours through absorbance reading at 450 nm Its optimized antibody pairing ensures high specificity for FAS quantification making it suitable for apoptosis-related research drug efficacy studies and biomarker validation across diverse biological matrices The assay s reliability and reproducibility have been validated under standard laboratory conditions