Biocolor Fastin Elastin Dye Dissociation Reagent Assay Component (3 x 30 ml) contains guanidine HCl and propan-1-ol Fastin Elastin Assay is a quantitative colorimetric dye-binding method for analysis of elastins released into tissue culture medium and extracted from biological materials It uses a 5 10 15 20-tetraphenyl-21H 23H-porphine tetra-sulfonate (TPPS) dye label Elastin forms that can be measured as -elastin soluble tropoelastins lathyrogenic elastins insoluble elastins (following solubilization to elastin polypeptides [ -elastin -elastin]) IN VITRO soluble elastin secreted into cell culture medium may be measured directly Tropoelastin is the elastin form usually found in medium during in vitro cell culture IN VIVO an extraction method of insoluble elastin from tissue and conversion to soluble -elastin or soluble -elastin is given After solubilization the samples are assayed by the procedure described for soluble elastin Sensitivity 5 g Assay Time 4 hrs