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Sigma Aldrich Fine Chemicals Biosciences FastStart PCR Master | 2x1.25ml
Supplier: Sigma Aldrich Fine Chemicals Biosciences 4710436001
Hot start polymerase chain reaction (PCR) is effortless using the FastStart PCR Master, a ready-to-use, double-concentrated hot start master mix. This master mix contains FastStart Taq DNA Polymerase, Roche high quality PCR-Grade nucleotides and all other reagents required for PCR and two-step reverse transcriptase (RT)-PCR on thermal cycler instruments. FastStart PCR Master accepts modified nucleotides such as Digoxigenin (DIG)-dUTP. Labeling with DIG-dUTP can be achieved by adding the modified nucleotide in a final concentration of 10 μM to the final volume.Combine this master mix with Roche′s Transcriptor First Strand cDNA Synthesis Kit to achieve excellent results in two-step RT-PCR.
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