Sigma Aldrich Fine Chemicals Biosciences fastStart SYBR Green Maste

Supplier:  Sigma Aldrich Fine Chemicals Biosciences 4673484001

fastStart SYBR Green Master Instructions for UsefastStart(TM) SYBR(R) Green Master is a ready-to-use hot start reaction mix without ROX for quantitative polymerase chain reaction (qPCR) and reverse transcription (RT)-qPCR on real-time PCR systems other than the LightCycler(R) instruments. This master mix simplifies the preparation of reactions for DNA detection and analysis. In combination with a real-time PCR instrument suitable PCR primers and a hydrolysis probe fastStart(TM) TaqMan(R) Probe Master allows very sensitive detection and quantification of defined DNA sequences.SYBR(R) Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis the SYBR(R) Green I dye included in the reaction mix binds to the amplified PCR products. The amplicon can be detected by its fluorescence.Hot start protocols have been shown to significantly improve the specificity sensitivity and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of fastStart(

Catalog No. 50-290-5950